How to Read a Semen Analysis Report?

Reading a semen analysis report is confusing, and this is completely normal. Sitting with a report, seeing numbers, abbreviations, and words like morphology or asthenozoospermia is kind of overwhelming. Also, it is difficult to understand what the semen analysis number means, right?

First, take a deep breath, and if you want to get clarification before going to the doctor, then make sure to read it correctly. 

This complete guide will include semen analysis results explained, what normal analysis values are, what semen analysis numbers mean, and also help you to know where your numbers stand. 

Important: As per WHO guidelines, one abnormal semen analysis does not define your fertility. Before drawing any conclusion, it is best to do a minimum of 2 tests, 4 to 1 week apart. 

Sheet of Parameters of Semen Analysis Report 

Parameters  Lower Limit Borderline Zone  Below Unit
Semen Volume >=1.4 mL 1.0- 1.4 mL <1.0 mL
Sperm Concentration >=16 million/ mL 10-16 million/ mL <10 million/ mL
Total Sperm Count  >=39 million 20-39 million  <20 million
Total motility (PR + NP) >=42% 35-42% <35%
Progressive motility (PR) >=30% 25-30% <25%
Morphology  >=4$ 3-4% <3%
Vitality (live sperm) >=54% 45-54% <45%
pH 7.2-8.0 <7.2 or > 8.0 
Liquefaction time  <60 minutes  >60 minutes 
WBCs/ Leukocytes <1 million/ mL >=1 million/ mL

Source: As per WHO’s 6th edition, Laboratory Manual for the Environment and Processing of Human Semen

Parameters of semen analysis

Let’s take a look at the parameter, which may help you to know about some concepts. It is based on the WHO 6th Edition Laboratory Manual (2021), and this is mostly used as a global gold standard used by fertility clinics.

Now you should know about each parameter specifically.

1. Semen Volume

As the name suggests, it is the volume of fluid collected or released during ejaculation. It typically ranges between 1.5 and 5 or 5.7 mL, or one-third to one full spoon.

For example:

Your report says, 

  • 1.4 mL or above – Normal. No concern with volume.
  • Low volume (below 1.4 mL)this is called hypsospermia, and it suggests that you have blocked seminal vesicles, retrograde ejaculation (semen partially entered the bladder), hormonal issues, or have a short abstinence period. 
  • If a semen volume is below 1.0mL, this is one of the biggest red flags and indicates the combination of volume with no sperm or absence of the vas deferens.

2. Sperm concentration

Sperm concentration defines how densely packed the sperm are in each millilitre of semen.

Based on the WHO’s larger dataset, the lower reference limit is 16 million sperm per millilitre, and a range above that is also considered normal. 

Also, the concentration is not a barrier to conception. 

But;

  • Range below 16 million/mL- not necessarily a problem, but it needs to be looked at by an expert to know about motility and morphology. 
  • Below 10 million/mL- also known as oligozoospermia, where sperm have a high concentration, indicating hormonal imbalance, varicocele (enlarged testicular veins), genetic condition, and many more. 
  • Zero sperm found- called azoospermia, where either a blockage happens which prevent sperm from reaching ejaculation or have problem. It is necessary to be treated. 

3. Motility

Men can have millions of sperm, but they will be unable to reach the egg if they are unable to move forward effectively. Motility is defined as the ability of the sperm to swim effectively. And it is divided into three types in the report:

  • PR – Progressive Motility: when sperm cells are able to move forward effectively.
  • NP – Non-Progressive Motility: Sperm cells that are moving, but not in the forward direction, i.e., they are circling, twitching, or making very slow non-linear movements.
  • IM – Immotile: Sperm cells that are not moving at all.

WHO 2021 Motility Reference Values

Progressive Motility (PR) – 30% or greater 

Total Motility (PR + NP) – 42% or greater

Your result shows…

If you have: PR is 30% or greater, and Total Motility is 42% or greater, then: Motility is normal. Sperm movement is normal.

If you have: Less than 30% in the above category, then: This indicates that you have asthenozoospermia. This is one of the most common semen analysis abnormalities. This can be due to improper nutrition, excessive smoking, or excessive alcohol intake.

4. Morphology 

This concept is majorly misinterpreted and least understood by men, and they worry about it the most. When men read ‘3% normal morphology,’ their first reaction is that 97% of their sperm are defective. This is not true and is not scary, and here is why:

Morphology is the shape of the sperm, including the shape of the head, shape of the middle piece, and shape of the tail, and it is graded by a detailed system called the Kruger strict criteria, where only a sperm with a very close to perfect shape passes the test.

Here is the thing that your report did not mention and that most people do not know: 

In an average fertile man, only 4-14% of their sperm have normal morphology. 

In fact, most of the sperm of men who have fathered babies are abnormal in shape. Because it is human biology.

As per the WHO 2021 report, 

The Lower Limit: ≥ 4% normal forms

Teratozoospermia is used to describe below 4%. 

This means that your morphology is not a problem.

5. pH, Liquefaction, and Viscosity

  • pH(acidity/alkalinity)- Semen naturally contains alkalines, and it protects sperm from the acidic environment of the vagina. Normal semen pH ranged between 7.2 and 8.0.
  • Liquefaction time- Semen is just like a gel, and it is supposed to be liquified within 15 to 60 minutes at room temperature, as it is driven by enzymes from the prostate. But if the liquefaction is unable to happen within 60 minutes, then it is defined as a thick gel, which reduces its ability to swim, secrete, and have poor motility. 
  • Viscosity-  This is considered normal when semen drops should fall as threads shorter than 2 cm. 
  • If this is thick and sticky semen, it shows that sperm motility is impaired, and there is seminal vesicle dysfunction. 

6. Vitality

Vitality can only be measured when the motility falls below 40%. At this point, it becomes crucial to know whether the sperm are dead or if they are not simply moving. 

According to WHO (World Health Organisation) standards, normal sperm vitality should be less than or equal to 54-58%, and it should be measured within an hour of ejaculation. 

7. WBCs and Round Cell

Round cells or leukocytes in semen are normal, and they can be immature, which is a sign of natural production. When WBCs exceed 1 million per mL, this is known as leukocytospermia, and it is a clear indication of infection or inflammation in the reproductive tract.

If these cells show an elevated number in the report, then the doctor recommend to go fo further urine and semen culture test. This should not be ignored, as it can lead to long-term damage to sperm quality. 

As per these numbers, you can analyse whether cells are mature or not, whether sperm are able to swim. And all these are done through a standard microscope. 

8. Total sperm count

This is equal to concentration and volume; in short, the actual number of sperm in the entire ejaculated sample. As per WHO, 39 million or above are consider as normal. But if your number starts to drop below this number, then this is a red flag that needs to be improved. 

What do borderline results indicate?

First of all, never rely on one test only, because the sperm parameter changes day to day. One week their result shoes good results, and another week they turn out to be bad. That is why, fertility experts suggest going for at least 2 to 3 tests to know the actual value of semen parameters. 

Now, when it comes to borderline, it sits between normal and abnormal. Sperm production is a complete 72-cycle process, and in between, if someone had stressful work, illness, heat exposure, or anything, it shows in the tests. If your month or weeks are stressful just before the test, then the test parameters might vary between borderline numbers. 

One abnormal test does not mean you are infertile. Let’s understand the situations which will give you an understanding of what semen analysis numbers mean, and the recommended next step. 

Situation   What does it really mean? What you should do. 
Parameters are within range. Your semen analysis is normal. Even after that, if the conception does not occur, try tracking your lifestyle factors. 
One parameter is below the range limit  Comes under borderline results or might be a temporary issue Go for analysis after 8 weeks of improving lifestyle. 
Multiple factors are below the limit.  Might be a sign of infertility. Talk to your doctor, know what the issues are, and implement the changes. 
Azoospermia (zero sperm) Can be obstructive or non-obstructive  Medical treatment is needed. 
Elevated WBCs Might be an infection or inflammation Antibodies can improve the infection. Go for a test after the treatment is done. 
Abnormal pH Congenital issue or ductal obstruction Go for a test and lifestyle improvement as the expert suggested. 

Important medical term that you should know

Let’s decode some medical terms-

  • Normozoospermia- normal parameters within the range.
  • Oligozoospermia- sperm concentration below 16 million/ mL.
  • Asthenozoospermia- progressive motility below 30%.
  • Teratozoozpermia- normal morphology below 4%.
  • Azoospemira- no sperm detected.
  • Necrozoospermia- high range of dead sperm.
  • Leukocytospermia- WBC count above 1 million/ mL.
  • Hypo- or oligospermia- semen volume below 1.4 mL.
  • Oligoasthenoteratozoospermia (OTA)- low count, motility and morphology.

One abnormal result doesn’t mean infertility — here’s what to do next

As of now, you must know that one result does not define whether you are fertile or not. You might have been exposed to heat or been ill, and this might have impacted your sperm morphology. Here are the things that you should know-

  • Sperm takes 72 hours to produce, and stressful moments, bad diet, tight clothing, antibiotics, etc., can impact it.
  • Even the tests taken 2 weeks apart with zero lifestyle changes can also show 20 to 30 % changes in morphology, count and motility. And this is also supported by the WHO.
  • It takes only one sperm to reach the egg, and roughly 7 million make the journey out of 16 million in one ejaculation. This describes probability, not possibility. 
  • A man with borderline range with a woman with a high fertile range can easily able to conceive. 
  • It requires at least 2 to 3 tests to know the actual possibility of fertility or infertility. 

Bottom line

Semen Analysis help to analyse the quality, quantity and movement of sperm in one sample. This guide might have given a sign on how you can understand your semen analysis report and also mentioned the term which are crucial. First of all, don’t panic, keep yourself calm. One result does not define whether you’re actually fertile or not. Before concluding anything, talk to an expert, and they can help you to know. Moreover, it is always best to adopt healthy habits, a healthy diet, and keep yourself away from heat exposure and chemicals. 

Aveya Fertility Centre is helping people to overcome their fear. So if you are also dealing with an issue related to your fertility, contact Aveya Fertility Centre. They are not just any centre, they are specialised in helping couples related to fertility, offer treatment options, guide them related to any concerns and also support through guidance. 

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