In Vitro Fertilization (IVF) is a multi-step assisted reproductive technology that has helped millions of couples conceive. Central to the IVF process are hormone injections, which stimulate the ovaries to produce multiple eggs, control ovulation timing, and prepare the uterus for embryo implantation. While IVF injections can seem overwhelming, understanding their types, timing, purpose, and administration can empower patients and improve treatment outcomes.
This comprehensive guide covers everything you need to know about IVF injections—from the start of the cycle to egg retrieval and beyond.
IVF injections are hormone medications administered via subcutaneous (under the skin) or intramuscular (into the muscle) routes. Their main goal is to regulate and optimize the reproductive cycle, ensuring the development of multiple mature eggs and preparing the body for successful fertilization and implantation.
Unlike a natural menstrual cycle, which typically produces one egg, IVF aims to stimulate the ovaries to produce multiple eggs to increase the chances of fertilization and embryo development.
These injections contain follicle-stimulating hormone (FSH), sometimes combined with luteinizing hormone (LH), to stimulate the growth and maturation of multiple ovarian follicles.
The goal is to produce multiple eggs to improve the chances of successful fertilization.
These hormones prevent premature ovulation by suppressing or blocking the body’s natural hormone surge.
The trigger shot is a critical injection that induces the final maturation of eggs and initiates ovulation.
After egg retrieval and embryo transfer, progesterone injections or vaginal suppositories support the uterine lining to facilitate embryo implantation and early pregnancy.
IVF injections usually begin on day 2 or 3 of your menstrual cycle, after your fertility specialist reviews your pre-cycle lab results and prescribes the stimulation medications. The exact start day and injection protocol depend on your individual fertility profile and treatment plan.
The entire injection phase can last from two to four weeks depending on your treatment cycle.
Most IVF injections are subcutaneous and can be self-administered at home. Your fertility clinic will provide detailed instructions and training on how to prepare and inject the medication safely.
Common injection sites:
Tips for self-injection:
Some medications, like progesterone in oil, require intramuscular injections, which may be more painful and sometimes require assistance.
Regular monitoring is essential to track your response to the injections and adjust dosages if needed. Monitoring typically involves:
Monitoring visits usually occur every 2-3 days during the stimulation phase.
The trigger shot is the most important injection in your IVF cycle. It causes the eggs to mature fully and prepares them for retrieval. Timing is critical—egg retrieval is scheduled 35 to 36 hours after the trigger shot to collect eggs before natural ovulation occurs.
Your fertility clinic will instruct you on the exact time to administer this injection. Missing or mistiming the trigger shot can affect egg maturity and retrieval success.
Common side effects during ovarian stimulation include:
Severe pain, persistent nausea, or symptoms of ovarian hyperstimulation syndrome (OHSS)—such as rapid weight gain, severe abdominal pain, or shortness of breath—require immediate medical attention.
The number of injections varies by individual and protocol, but generally includes:
In some cases, women may receive up to 90 injections throughout the entire IVF cycle, especially if multiple medications and protocols are involved.
| Stage | Duration/Timing | Purpose |
| Ovarian Stimulation | 8-14 days | Stimulate multiple follicles |
| GnRH Agonist/Antagonist | Concurrent with stimulation | Prevent premature ovulation |
| Trigger Shot | Single injection, ~36 hours before egg retrieval | Final egg maturation |
| Luteal Phase Support | Several weeks post-embryo transfer | Support the uterine lining for implantation |
IVF injections are essential to the success of the IVF process, designed to maximize egg production, control ovulation timing, and support early pregnancy. While the number and complexity of injections can seem daunting, understanding their purpose and schedule can help reduce anxiety and improve compliance.
Our fertility care team at Aveya IVF centre will provide personalized instructions, monitoring, and support to guide you through each step. With proper preparation and care, IVF injections can be managed safely and effectively, bringing you closer to your goal of parenthood.